Our Team

KARAN SARAT S202
KJ SHAKTI S202
GOH JIA SHENG S202

Overview of Project

• What is the theory/ concept behind the topic?

Our "Mission"

• What does the group need to accomplish at the end of the workshop

Our "Mission" Part 2(i)

6.1 Histochemical Staining(Questions from Project Manual)

• 1) What are the colours of the stained tissue?
• 2) What is the pattern of colour distribution in the stained tissue?
• 3) Can you identify which are the nuclei of the cells? What colour are they?
• 4) Can you identify the cytoplasm of the cells? What colour are they?
• 5) What are the relative sizes and shapes of the cells in the tissue?
• 6) How many different types of cells do you think there are in the tissue?
• 7) Are there any other observations you have identified? If so, describe them.

Our "Mission" Part 2(ii)

6.2 Immunohistochemical Staining(Questions from Project Manual)

• What are the colours in the tissue?
• What is the pattern of colour distribution in the stained tissue?
• Are the colours and pattern of colour distribution similar to that in the first slide?
• The purpose was to determine the presence and location/distribution of a protein called
• vimentin. Which colour do you think represents vimentin? Why?
• Describe the location/distribution of vimentin in the tissue.
• What does the other colour(s) represent?
• Are there any other observations you have identified? If so, describe them.

Our "Mission" Part 2(iii)

6.3 Fluorescence Staining(Questions from Project Manual)

• Can you see the tissue using the fluorescence microscope? Does the tissue appear
• similar to the first and second slide?
• Describe what you observe. How many colours appear when using the fluorescence
• microscope and what are the colours?
• What do you think the colours represent? Why?
• Can you determine what is/are the pattern of location/distribution of the colours in the
• tissue? If no, why not?
• Are there any other observations you have identified? If so, describe them.

Our Accomplishment

• What is the end product/ findings?
• A description/ explanation of your product/ findings

Our Gains...

• As a group,
• discuss how the project has helped to deepen your understanding/ broaden your awareness on selected subject discipline(s)
• describe how the new learning is/ can be connected to what you learnt/ are going to learn